High-yield expression of recombinant SARS coronavirus nucleocapsid protein in methylotrophic yeast Pichia pastoris.
Identifieur interne : 005323 ( Main/Exploration ); précédent : 005322; suivant : 005324High-yield expression of recombinant SARS coronavirus nucleocapsid protein in methylotrophic yeast Pichia pastoris.
Auteurs : Ru-Shi Liu [République populaire de Chine] ; Kun-Yu Yang ; Jian Lin ; Yi-Wei Lin ; Zhi-Hong Zhang ; Jun Zhang ; Ning-Shao XiaSource :
- World journal of gastroenterology [ 1007-9327 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN complémentaire, Escherichia coli (génétique), Milieux de culture (métabolisme), Milieux de culture (pharmacologie), Méthanol (métabolisme), Méthanol (pharmacologie), Phénotype, Pichia (croissance et développement), Pichia (génétique), Pichia (métabolisme), Plasmides (génétique), Protéines nucléocapside (génétique), Protéines recombinantes (génétique), Techniques bactériologiques, Transformation génétique, Virus du SRAS (génétique).
- MESH :
- croissance et développement : Pichia.
- génétique : Escherichia coli, Pichia, Plasmides, Protéines nucléocapside, Protéines recombinantes, Virus du SRAS.
- métabolisme : Milieux de culture, Méthanol, Pichia.
- pharmacologie : Milieux de culture, Méthanol.
- ADN complémentaire, Phénotype, Techniques bactériologiques, Transformation génétique.
English descriptors
- KwdEn :
- Bacteriological Techniques, Culture Media (metabolism), Culture Media (pharmacology), DNA, Complementary, Escherichia coli (genetics), Methanol (metabolism), Methanol (pharmacology), Nucleocapsid Proteins (genetics), Phenotype, Pichia (genetics), Pichia (growth & development), Pichia (metabolism), Plasmids (genetics), Recombinant Proteins (genetics), SARS Virus (genetics), Transformation, Genetic.
- MESH :
- chemical , genetics : Nucleocapsid Proteins, Recombinant Proteins.
- chemical , metabolism : Culture Media, Methanol.
- chemical , pharmacology : Culture Media, Methanol.
- genetics : Escherichia coli, Pichia, Plasmids, SARS Virus.
- growth & development : Pichia.
- metabolism : Pichia.
- Bacteriological Techniques, DNA, Complementary, Phenotype, Transformation, Genetic.
Abstract
Nucleocapsid (N) protein plays an important role in reproduction and pathological reaction of severe acute respiratory syndrome (SARS) coronavirus (SCoV), the antigenicity of the protein is better than spike (S) protein. This study was to find a highly specific and antigenic recombinant SCoV nucleocapsid (rSCoVN) protein, and to provide a basis for further researches on early diagnosis of SARS.
DOI: 10.3748/wjg.v10.i24.3602
PubMed: 15534914
Affiliations:
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Le document en format XML
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The Research Center for Medical Molecular Virology of Fujian Province, Xiamen University, Xiamen 361005, Fujian Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>The Key Laboratory for Cell Biology and Tumor Cell Engineering of the Ministry of Education; The Research Center for Medical Molecular Virology of Fujian Province, Xiamen University, Xiamen 361005, Fujian Province</wicri:regionArea><wicri:noRegion>Fujian Province</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Kun Yu" sort="Yang, Kun Yu" uniqKey="Yang K" first="Kun-Yu" last="Yang">Kun-Yu Yang</name></author><author><name sortKey="Lin, Jian" sort="Lin, Jian" uniqKey="Lin J" first="Jian" last="Lin">Jian Lin</name></author><author><name sortKey="Lin, Yi Wei" sort="Lin, Yi Wei" uniqKey="Lin Y" first="Yi-Wei" last="Lin">Yi-Wei Lin</name></author><author><name sortKey="Zhang, Zhi Hong" sort="Zhang, Zhi Hong" uniqKey="Zhang Z" first="Zhi-Hong" last="Zhang">Zhi-Hong Zhang</name></author><author><name sortKey="Zhang, Jun" sort="Zhang, Jun" uniqKey="Zhang J" first="Jun" last="Zhang">Jun Zhang</name></author><author><name sortKey="Xia, Ning Shao" sort="Xia, Ning Shao" uniqKey="Xia N" first="Ning-Shao" last="Xia">Ning-Shao Xia</name></author></analytic><series><title level="j">World journal of gastroenterology</title><idno type="ISSN">1007-9327</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteriological Techniques</term><term>Culture Media (metabolism)</term><term>Culture Media (pharmacology)</term><term>DNA, Complementary</term><term>Escherichia coli (genetics)</term><term>Methanol (metabolism)</term><term>Methanol (pharmacology)</term><term>Nucleocapsid Proteins (genetics)</term><term>Phenotype</term><term>Pichia (genetics)</term><term>Pichia (growth & development)</term><term>Pichia (metabolism)</term><term>Plasmids (genetics)</term><term>Recombinant Proteins (genetics)</term><term>SARS Virus (genetics)</term><term>Transformation, Genetic</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire</term><term>Escherichia coli (génétique)</term><term>Milieux de culture (métabolisme)</term><term>Milieux de culture (pharmacologie)</term><term>Méthanol (métabolisme)</term><term>Méthanol (pharmacologie)</term><term>Phénotype</term><term>Pichia (croissance et développement)</term><term>Pichia (génétique)</term><term>Pichia (métabolisme)</term><term>Plasmides (génétique)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines recombinantes (génétique)</term><term>Techniques bactériologiques</term><term>Transformation génétique</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Culture Media</term><term>Methanol</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Culture Media</term><term>Methanol</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Pichia</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Pichia</term><term>Plasmids</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Pichia</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term><term>Pichia</term><term>Plasmides</term><term>Protéines nucléocapside</term><term>Protéines recombinantes</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Pichia</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Milieux de culture</term><term>Méthanol</term><term>Pichia</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Milieux de culture</term><term>Méthanol</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Bacteriological Techniques</term><term>DNA, Complementary</term><term>Phenotype</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>Phénotype</term><term>Techniques bactériologiques</term><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nucleocapsid (N) protein plays an important role in reproduction and pathological reaction of severe acute respiratory syndrome (SARS) coronavirus (SCoV), the antigenicity of the protein is better than spike (S) protein. This study was to find a highly specific and antigenic recombinant SCoV nucleocapsid (rSCoVN) protein, and to provide a basis for further researches on early diagnosis of SARS.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Lin, Jian" sort="Lin, Jian" uniqKey="Lin J" first="Jian" last="Lin">Jian Lin</name><name sortKey="Lin, Yi Wei" sort="Lin, Yi Wei" uniqKey="Lin Y" first="Yi-Wei" last="Lin">Yi-Wei Lin</name><name sortKey="Xia, Ning Shao" sort="Xia, Ning Shao" uniqKey="Xia N" first="Ning-Shao" last="Xia">Ning-Shao Xia</name><name sortKey="Yang, Kun Yu" sort="Yang, Kun Yu" uniqKey="Yang K" first="Kun-Yu" last="Yang">Kun-Yu Yang</name><name sortKey="Zhang, Jun" sort="Zhang, Jun" uniqKey="Zhang J" first="Jun" last="Zhang">Jun Zhang</name><name sortKey="Zhang, Zhi Hong" sort="Zhang, Zhi Hong" uniqKey="Zhang Z" first="Zhi-Hong" last="Zhang">Zhi-Hong Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Ru Shi" sort="Liu, Ru Shi" uniqKey="Liu R" first="Ru-Shi" last="Liu">Ru-Shi Liu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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